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Addgene inc usp2258
Usp2258, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/usp2258/Flag-HA-USP2+(Plasmid+%2322577)/pm37443395-231-59-60
Average 91 stars, based on 6 article reviews
usp2258 - by Bioz Stars, 2026-09
91/100 stars

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Clone Assay:

Article Title: Molecular basis for ubiquitin/Fubi cross-reactivity in USP16 and USP36.
Article Snippet: Human Fubi1–73, HA–Fubi1–73, GST–3C-GS-HA–Fubi1–73, ubiquitin1–75, HA– ubiquitin1–75, HA–NEDD81-75, HA–ISG151–156, HA–UFM11–82, HA–URM11–100 and HA–SUMO11–96 were cloned from Escherichia coli optimized DNA gene strings into pTXB1 using restriction enzymes. cDNA encoding catalytic domains of human USP16191–823 (Medical Research Council Protein Phosphorylation and Ubiquitylation (MRC PPU), DU25374), USP4270–446 (MRC PPU, DU15140), USP3681–461 and USP3681–424 (MRC PPU, DU49030) were cloned into pOPINK, and USP2258–605 (Addgene, 22577), USP8734–1110 (Addgene, 22608) and USP1816–372 (MRC PPU, DU14320) were cloned into pOPINB using an In-Fusion HD cloning kit (Takara Clonetech). .. Human Fubi1–73, HA–Fubi1–73, GST–3C-GS-HA–Fubi1–73, ubiquitin1–75, HA– ubiquitin1–75, HA–NEDD81-75, HA–ISG151–156, HA–UFM11–82, HA–URM11–100 and HA–SUMO11–96 were cloned from Escherichia coli optimized DNA gene strings into pTXB1 using restriction enzymes. cDNA encoding catalytic domains of human USP16191–823 (Medical Research Council Protein Phosphorylation and Ubiquitylation (MRC PPU), DU25374), USP4270–446 (MRC PPU, DU15140), USP3681–461 and USP3681–424 (MRC PPU, DU49030) were cloned into pOPINK, and USP2258–605 (Addgene, 22577), USP8734–1110 (Addgene, 22608) and USP1816–372 (MRC PPU, DU14320) were cloned into pOPINB using an In-Fusion HD cloning kit (Takara Clonetech). .. USP451–814 was expressed from a pGEX6P vector (MRC PPU, DU15681).

Phospho-proteomics:

Article Title: Molecular basis for ubiquitin/Fubi cross-reactivity in USP16 and USP36.
Article Snippet: Human Fubi1–73, HA–Fubi1–73, GST–3C-GS-HA–Fubi1–73, ubiquitin1–75, HA– ubiquitin1–75, HA–NEDD81-75, HA–ISG151–156, HA–UFM11–82, HA–URM11–100 and HA–SUMO11–96 were cloned from Escherichia coli optimized DNA gene strings into pTXB1 using restriction enzymes. cDNA encoding catalytic domains of human USP16191–823 (Medical Research Council Protein Phosphorylation and Ubiquitylation (MRC PPU), DU25374), USP4270–446 (MRC PPU, DU15140), USP3681–461 and USP3681–424 (MRC PPU, DU49030) were cloned into pOPINK, and USP2258–605 (Addgene, 22577), USP8734–1110 (Addgene, 22608) and USP1816–372 (MRC PPU, DU14320) were cloned into pOPINB using an In-Fusion HD cloning kit (Takara Clonetech). .. Human Fubi1–73, HA–Fubi1–73, GST–3C-GS-HA–Fubi1–73, ubiquitin1–75, HA– ubiquitin1–75, HA–NEDD81-75, HA–ISG151–156, HA–UFM11–82, HA–URM11–100 and HA–SUMO11–96 were cloned from Escherichia coli optimized DNA gene strings into pTXB1 using restriction enzymes. cDNA encoding catalytic domains of human USP16191–823 (Medical Research Council Protein Phosphorylation and Ubiquitylation (MRC PPU), DU25374), USP4270–446 (MRC PPU, DU15140), USP3681–461 and USP3681–424 (MRC PPU, DU49030) were cloned into pOPINK, and USP2258–605 (Addgene, 22577), USP8734–1110 (Addgene, 22608) and USP1816–372 (MRC PPU, DU14320) were cloned into pOPINB using an In-Fusion HD cloning kit (Takara Clonetech). .. USP451–814 was expressed from a pGEX6P vector (MRC PPU, DU15681).

Cloning:

Article Title: Molecular basis for ubiquitin/Fubi cross-reactivity in USP16 and USP36.
Article Snippet: Human Fubi1–73, HA–Fubi1–73, GST–3C-GS-HA–Fubi1–73, ubiquitin1–75, HA– ubiquitin1–75, HA–NEDD81-75, HA–ISG151–156, HA–UFM11–82, HA–URM11–100 and HA–SUMO11–96 were cloned from Escherichia coli optimized DNA gene strings into pTXB1 using restriction enzymes. cDNA encoding catalytic domains of human USP16191–823 (Medical Research Council Protein Phosphorylation and Ubiquitylation (MRC PPU), DU25374), USP4270–446 (MRC PPU, DU15140), USP3681–461 and USP3681–424 (MRC PPU, DU49030) were cloned into pOPINK, and USP2258–605 (Addgene, 22577), USP8734–1110 (Addgene, 22608) and USP1816–372 (MRC PPU, DU14320) were cloned into pOPINB using an In-Fusion HD cloning kit (Takara Clonetech). .. Human Fubi1–73, HA–Fubi1–73, GST–3C-GS-HA–Fubi1–73, ubiquitin1–75, HA– ubiquitin1–75, HA–NEDD81-75, HA–ISG151–156, HA–UFM11–82, HA–URM11–100 and HA–SUMO11–96 were cloned from Escherichia coli optimized DNA gene strings into pTXB1 using restriction enzymes. cDNA encoding catalytic domains of human USP16191–823 (Medical Research Council Protein Phosphorylation and Ubiquitylation (MRC PPU), DU25374), USP4270–446 (MRC PPU, DU15140), USP3681–461 and USP3681–424 (MRC PPU, DU49030) were cloned into pOPINK, and USP2258–605 (Addgene, 22577), USP8734–1110 (Addgene, 22608) and USP1816–372 (MRC PPU, DU14320) were cloned into pOPINB using an In-Fusion HD cloning kit (Takara Clonetech). .. USP451–814 was expressed from a pGEX6P vector (MRC PPU, DU15681).



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Addgene inc usp2258
Usp2258, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/usp2258/Flag-HA-USP2+(Plasmid+%2322577)/pm37443395-231-59-60
Average 91 stars, based on 1 article reviews
usp2258 - by Bioz Stars, 2026-09
91/100 stars
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